Publication
Androgenic activation, impairment of the monoaminergic system and altered behavior in zebrafish larvae
Journal Article (2021)
Journal
Science of the Total Environment
Pages
145671
Volume
775
Doc link
https://doi.org/10.1016/j.scitotenv.2021.145671
File
Authors
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Faria, Melissa
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Prats, Eva
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Rosas Ramírez, Jonathan Ricardo
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Bellot, Marina
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Bedrossiantz, Juliette
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Pagano, Maria
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Valls Brusco, Arnau
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Gomez Canela, Cristian
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Porta Pleite, Josep Maria
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Mestres, Jordi
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Garcia Reyero, Natalia
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Faggio, Caterina
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Gómez Oliván, Leobardo Manuel
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Raldúa, Demetrio
Abstract
Fenitrothion is an organophosphorus insecticide usually found in aquatic ecosystems at concentrations in the range of low ng/L. In this manuscript we show that 24 h exposure to environmental concentrations of fenitrothion, from ng/L to low μg/L, altered basal locomotor activity, visual-motor response and acoustic/vibrational escape response of zebrafish larvae. Furthermore, fenitrothion and expression of gap43a, gfap, atp2b1a, and mbp exhibited a significant non-monotonic concentration-response relationship. Once determined that environmental concentrations of fenitrothion were neurotoxic for zebrafish larvae, a computational analysis identified potential protein targets of this compound. Some of the predictions, including interactions with acetylcholinesterase, monoamine-oxidases and androgen receptor (AR), were experimentally validated. Binding to AR was the most suitable candidate for molecular initiating event, as indicated by both the up-regulation of cyp19a1b and sult2st3 and the non-monotonic relationship found between fenitrothion and the observed responses. Finally, when the integrity of the monoaminergic system was evaluated, altered levels of L-DOPA, DOPAC, HVA and 5-HIAA were found, as well as a significant up-regulation of slc18a2 expression at the lowest concentrations of fenitrothion. These data strongly suggest that concentrations of fenitrothion commonly found in aquatic ecosystems present a significant environmental risk for fish communities.
Categories
automation.
Author keywords
Neurotoxicity; Predicted target profile; Acetylcholinesterase inhibitor; Endocrine disruptors; Androgenic effects; Risk assessment
Scientific reference
M. Faria, E. Prats, J.R. Rosas, M. Bellot, J. Bedrossiantz, M. Pagano, A. Valls, C. Gomez-Canela, J.M. Porta, J. Mestres, N. Garcia-Reyero, C. Faggio, L.M. Gómez and D. Raldúa. Androgenic activation, impairment of the monoaminergic system and altered behavior in zebrafish larvae. Science of the Total Environment, 775: 145671, 2021.
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